Screening for canine coronavirus, canine influenza virus, and severe acute respiratory syndrome coronavirus 2 in dogs during the coronavirus disease-2019 pandemic.

Background and Aim: Although most cases of coronavirus disease-2019 (COVID-19) are in humans, there is scientific evidence to suggest that the virus can also infect dogs and cats. This study investigated the circulation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), canine coronavirus (CCV), and canine influenza virus (CIV) in domiciled and/or stray dogs from different locations in the State of Minas Gerais, Brazil, during the COVID-19 pandemic. Materials and Methods: In total, 86 dogs living in homes, on the streets, or in shelters in the cities of Taiobeiras, Salinas, Araçuaí, and Almenara were randomly selected for this study. The COVID Ag Detect® Self-Test was used to detect SARS-CoV-2. The ACCUVET CCV AG TEST - CANINE CORONAVIROSIS® was used to detect CCV, whereas canine influenza was detected using the ACCUVET CIV AG TEST - INFLUENZA CANINA®. All collected data were mapped using QGIS 3.28.1 for spatial data analysis and the identification of disease distribution patterns. Descriptive analysis of the collected data, prevalence calculations, odds ratios (ORs), and 95% confidence intervals, when possible, was performed. Results: Of the 86 animals tested, only one dog tested positive for SARS-CoV-2 using the rapid test for viral antigen detection. No animals tested positive for CIV. Canine coronavirus was detected in almost half of the animals tested in Almenara. Severe acute respiratory syndrome-CoV-2 had a low prevalence (1.16%), versus 15.62% for CCV. Although the results were not significant, the age and breed of animals appeared to be associated with the occurrence of CCV. The results indicated that younger animals were 2.375-fold more likely to be infected. Likewise, purebred animals were more likely to contract the disease (OR = 1.944). Conclusion: The results indicate the need to maintain preventive measures against CCV, canine influenza, and SARS-CoV-2 in dogs. More studies are needed to better elucidate the panorama of these diseases in dogs, mainly in underdeveloped and developing countries.

Contagem de microrganismos indicadores em queijo cozido produzido em Salinas-MG / Indicator microorganisms count in cooked cheese produced in the city of Salinas-MG

Na região de Salinas, assim como no estado de Minas Gerais inteiro, a fabricação e comercialização de queijo artesanal é um hábito cultural. A venda de queijo em feira comercial ou em residências é uma prática tradicional que oferece sustento para muitos produtores da cidade mineira. O presente estudo aborda a qualidade de queijo cozido produzido por produtores da região de Salinas-MG. Foram coletadas amostras de queijos já prontos para consumo, de 10 produtores escolhidos aleatoriamente no Mercado Municipal da cidade. As amostras foram analisadas no laboratório de microbiologia do Instituto Federal do Norte de Minas Gerais (IFNMG) - Campus Salinas. Nas análises realizadas foi possível notar uma alta contagem de mesófilos e psicrotróficos, e baixa contagem de coliformes totais e termotolerantes, com três amostras fora dos padrões legais. Sugere -se ações de capacitação e regulamentação pelo poder público para melhorar a qualidade final do produto.